Bioanalysis of the investigational anti-tumour drug 5,10-dideaza-5,6,7,8-tetrahydrofolic acid by HPLC with UV detectionon
Summary
Type: HPLC Application #: 104450
Detection: UV, 278nm, 0.002 AUFS Conditions: eluent: 803ml 50mM KH2PO4 adjusted to pH 3.5 with HCl, 40ml PIC B-8 reagent, 157ml acetonitrile flow rate 0.4ml/min Authors: van Tellingen, O. et al. Source: J. Chromatogr. 576 (1992) 158–162
Details
Legend: Fig. 2. Chromatograms of A) blank human plasma, B) human plasma spiked with
100ng/ml DDATHF, C) rat plasma obtained 6h and D) rat plasma obtained 5min
after administration of 4mg/kg DDATHF, 1. metho